Industry Recognized
Gold Standard for Uniform Natural Killer Cell Bioassays
IDEAL SOLUTIONS
COMMON APPLICATIONS
Fill Out Request Form
Receive, Review, and Sign NDA
Review Term Sheet for License
Discuss Details of License, Finalize, Sign and Pay Fees
Obtain Cells Under License
Receive Technical Support & Research Services
Neukopanel® Cell Request Form
for Proprietary Neukoplast® NK-92® Cells
Please complete form to initiate licensing process
Innate Binding
aNK cells surveil each cell they encounter and are naturally equipped to recognize and bind to stress ligands expressed on cancer and virally infected cell surfaces via an array of innate receptors.
Activation
Upon engagement, aNK cells undergo cytolytic granule polarization towards the cell synapse in preparation for mass fusion with the membrane.
Degranulation
Cytolytic granules discharge their perforin, granzyme and cytokine contents into the synapse for direct exposure to the targeted cells.
Killing
Perforins punch holes in the cellular and nuclear membranes, allowing granzymes to enter and degrade the target cells from the inside. Destructive enzymes break down membrane structures and the internal machinery, including the nucleus with its genetic code. The targeted cells swell and rupture, leaving only debris behind.
Antibody Binding
Monoclonal antibodies bind to unique surface proteins on target cells, which in turn bind to CD16 receptors on haNK cells
Activation
Upon engagement, haNK cells undergo polarization and migration of cytolytic granules towards the cell synapse in preparation for mass fusion with the membrane.
Degranulation
Cytolytic granules discharge their perforin, granzyme and cytokine contents into the synapse for direct exposure to the targeted cells.
Killing
Perforins punch holes in the cellular and nuclear membranes, allowing granzymes to enter and degrade the target cells from the inside. Destructive enzymes break down membrane structures and the internal machinery, including the nucleus with its genetic code. The targeted cells swell and rupture, leaving only debris behind.
Epitope Binding
taNK cells bind directly to unique surface proteins on targeted cells via their CAR receptors.
Activation
Once bound, the transmembrane portion of these CAR receptors relay activation signals to the cytoplasm, initiating a cascade of events leading to the migration and polarization of cytolytic granules towards the cell synapse.
Degranulation
These granules fuse with the membrane surface and discharge their perforin, granzyme and cytokines into synapse for direct exposure to the targeted cells.
Killing
Perforins punch holes in the cellular and nuclear membranes, allowing granzymes to enter and degrade the target cells from the inside. Destructive enzymes break down membrane structures and the internal machinery, including the nucleus with its genetic code. The targeted cells swell and rupture, leaving only debris behind.
aNK™, MI-aNK™, haNK®, laNK™, and taNK® Informational and Technical Support Research Services
aNK™ Cells
direct killing using NK-92 derived activated NK cells (aNK) that release toxic granules directly into the cancer cells through cell-to-cell contact.
MI-aNK™ Cells
Direct killing using NK-92 transfected with human IL-2 cDNA in the retroviral MFG-hIL-2 vector that release toxic granules directly into the cancer cells through cell-to-cell contact.
haNK® Cells
Antibody-mediated killing using haNKs, cells which are NK cells engineered to incorporate a high binding affinity receptor that binds to an administered antibody, designed to enhance the cancer cell killing effect of that antibody.
laNK™ Cells
Antibody-mediated killing using haNKs, cells which are NK cells engineered to incorporate a low binding affinity receptor that binds to an administered antibody, designed to enhance the cancer cell killing effect of that antibody.
taNK® Cells
Chimeric Antigen Receptor directed killing using taNKs, cells which are NK cells engineered to incorporate chimeric antigen receptors (CARs) to target tumor-specific antigens found on the surface of cancer cells.
Taking you beyond the Brink of binding®
with our Killer Assays™.
THE NK-92® ASSAY COMPANY
Brink Biologics, Inc. (BBI) is a ImmunityBio, Inc. (NASDAQ: IBRX) affiliated biotechnology solutions company located in the prestigious Torrey Pines biotechnology science park in San Diego, CA.
KILLER CELLS - KILLER ASSAYS™
Natural Killer cell lines originating from a singular source, established in stable cultures under GLP & cGMP conditions and further modified into multiple panels of lines suitable for a range of laboratory testing applications.
Proprietary NEUKOPANEL® CELL LINES
A standardized panel of CD16 expressing NK-92® cell lines, provides for the first time the means to establish a consistent, robust and reliable assay for assessment of ADCC activity.
ADCC ASSAYS – ROUTINE HASSLE
Brink Biologics, Inc. holds the exclusive non-clinical rights to the NK-92 cell line and its CD16 expressing derivatives, all of which are uniquely and ideally suited for antibody-dependent cell-mediated cytotoxicity (ADCC) assays.
OUR SIMPLE SOLUTION
The exclusive global provider of THE NK-92 cell line (AND ITS VARIANTS) AND THE NEUKOPANELtesting services, NEUKOPANEL CELL PANELS, and sublicenses within the life sciences and device industry.
Highly published cell lines – over 200 publications.
Relevant Examples:
Schnueriger, A., et al. (2011). “Development of a quantitative, cell-line based assay to measure ADCC activity mediated by therapeutic antibodies.” Mol Immunol 48(12-13): 1512-1517.
NK-92 cells are the only continuously growing human NK cell adapted to mass production
Brink Biologics created the NK-92 master cell bank (MCB).
Five Advantages of Neukopanel Cell Lines:
For the first time the means to establish a consistent, robust and reliable assay for assessment of true ADCC function.
Cost & Time Savings
True Cytotoxicity
Ease of Use
Robust Assay
Eliminates Phenotypic Variability
- Cost and time savings over common practice using donor-sourced (PBMC) NK cell isolation, expansion and maintenance in short term cultures
- Eliminates phenotypic variability of donor-sourced NK cells as Neukopanel cell lines offer clonal consistency, purity and reliability as an assay reagent
- Ease of use over donor-sourced NK cells and capable of long-term continuous cultures
- Ability to generate a readout spectrum with the use of our homozygous high and low affinity CD16 expressing lines alongside our negative control line for assay robustness
- True cytotoxicity rather than cell-binding readouts
Office
3530 John Hopkins Court
San Diego, CA 92121
Hours
8am to 5pm M-F
Call Us
Main: (858) 633-0300
Fax: (858) 380-1999
Email: info@brinkbiologics.com
©2015-2025 Brink Biologics, Inc. All Rights Reserved | Home | Privacy Policy | Legal Notice | Patent Notice
The content of this website is protected by Title 17 of the U.S. Code and may not be reproduced in whole or in part by any means.
